Monoclonal antibody-based serological methods for detecting Citrus tristeza virus in citrus groves
نویسندگان
چکیده
منابع مشابه
Citrus tristeza virus-host interactions
Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes sev...
متن کاملSurvey methods for assessment of citrus tristeza virus incidence.
ABSTRACT The assessment of citrus tristeza virus incidence by sampling involves laboratory testing by enzyme-linked immunosorbent assay of leaf material collected in the field. Using field data and computer simulation, methods of field data collection were compared. One method was similar to that used by the Central California Tristeza Eradication Agency, in which 4 to 6% of the trees in a plan...
متن کاملThe Complex Genetics of Citrus tristeza virus
The 2000 x 11 nm long bipolar flexuous filamentous particles of Citrus tristeza virus (CTV) (genus Closterovirus, family Closteroviridae) (Figure 1) contain a single-stranded positive-sense RNA genome of 19.3 kb, which is encapsidated in two different capsid proteins that coat the opposite ends of the virions [1, 2]. CTV is the largest identified RNA virus infecting plants and the second larges...
متن کاملProduction and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained from hybrid cells produced by fusion of a non-secreting myeloma cell line with spleen cells from BALB/c mice immunized with isolate T-308 of CTV. Three MAb were characterized for their immunoglobulin isotype and their titres in cell culture and ascites fluids. Each MAb was conjugated with alkaline phosphatase and...
متن کاملEpidemiology of Citrus tristeza virus in nursery blocks of Citrus macrophylla and evaluation of control measures
The control of Citrus tristeza virus (CTV), the causal agent of tristeza disease, is essential to guarantee a productive citrus industry. The specific features of a nursery block make the control of the natural spread of viruses transmitted by vectors more difficult. Thus, the knowledge of the epidemiology of CTV in nursery blocks is basic to the design control strategies of the disease. Two ex...
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ژورنال
عنوان ژورنال: Virologica Sinica
سال: 2016
ISSN: 1674-0769,1995-820X
DOI: 10.1007/s12250-016-3718-4